GFAP antibody [GFAP/2076]

Cat. No. GTX18062

Host	Mouse
Clonality	Monoclonal
Clone Name	GFAP/2076
Isotype	IgG1
Application	WB, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

See all GFAP products

Datasheet
Document

View product citations for antibody GTX18062 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GFAP protein fragment (around aa 101-200) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ALXDRD , GFAP , glial fibrillary acidic protein

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Gene ID: 2670 GFAP
UniProt: P14136 GFAP

Research Area

DATA IMAGES

GTX18062 FACS Image

FACS analysis of T98G cells using GTX18062 GFAP antibody [GFAP/2076].
Blue : Primary antibody
Red : Isotype control

GTX18062 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX18062 GFAP antibody [GFAP/2076].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty